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. 2023 Apr 10;14:1094359. doi: 10.3389/fphys.2023.1094359

FIGURE 3.

FIGURE 3

Severe and progressive muscle changes in the heart of DMD nude rats. (A) Representative images of Sirius red (SR)-stained hearts of WT and DMD nude rats, showing the degree of fibrosis at each respective age. WT nude rats are shown on the left, and DMD nude rats are shown on the right. Scale bar = 1 mm (one-month-old), scale bar = 500 µm (5- and 12- month-old) (B) Detailed analysis of severe and progressive changes in the heart of a DMD nude rat. The upper row shows hematoxylin & eosin (H&E) staining, and the lower row shows SR staining images. The right panel shows magnified views of the left panel. Magnified area denoted by the square in panel (A,B) ×4, scale bar = 500 μm; ×10, scale bar = 50 µm. (C) Bar graph summarizing SR-positive areas in WT and DMD nude rats at 1, 5, and over 12 months of age. N.S. non-significant, *p < 0.05, **p < 0.01.