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. 2023 Apr 18;64(4):20. doi: 10.1167/iovs.64.4.20

Figure 8.

Figure 8.

Cells in meridional rows exhibit aberrant cell shapes and irregular packing in NMIIAE1841K/E1841K lenses. (A) F-actin staining in single optical sections at the basal region of meridional row cells in NMIIA+/+ and NMIIAE1841K/E1841K lenses, shown in XY plane. F-actin is enriched along all six sides of meridional row epithelial cells. Scale bar, 20 µm. Yellow boxes, regions enlarged in (B). (B) High magnification of a region from A (yellow boxes), showing an individual cell (C) (central cell) surrounded by six neighboring cells (numbered) in the NMIIA+/+ lens, but surrounded by seven neighboring cells in the NMIIAE1841K/E1841K lens. Scale bar, 10 µm. (C) Frequency distribution (%) of the number of adjacent cells for 200 cells from 5 different lenses. Almost all cells in NMIIA+/+ lenses have six nearest neighbors, whereas cells in NMIIAE1841K/E1841K lenses have variable numbers of nearest neighbors (4–8). (D) Percentage of hexagonal adjacent cells in NMIIA+/+ and NMIIAE1841K/E1841K lenses. The plot represents the mean ± SD of five different lens images from at least four different mice. *P < 0.05.