Figure 2.

YY1 induced endogenous Msx2 expression in P19 embryonal cells. Different amounts of YY1 (x-axis) were transfected into P19 cells cultured on 10-cm plates, and Msx2 expression level was assayed by semi-quantitative RT–PCR 24 h post-transfection. Increasing amounts of YY1 resulted in significantly elevated levels of Msx2 when compared with control in which an expression vector with no YY1 insert (empty vector) was used. *P < 0.01.