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. 2002 Mar 1;30(5):1213–1223. doi: 10.1093/nar/30.5.1213

Figure 6.

Figure 6

BMP4-soaked beads induced Msx2 but not YY1 expression. Mandibular processes were isolated from E10 mouse embryos and explanted in organ culture. Affi-gel microbeads with a diameter of 100–150 µm were soaked in PBS as control (A), or 100 ng/µl BMP4 (B–E) and implanted into mandibular explants in the medial (black arrowheads) and lateral (gray arrowheads) positions. At different time points indicated on the left, explants were probed by whole mount in situ hybridization for YY1 (A–D) or Msx2 (E). Endogenous expression of these genes was detected in all explants. Neither PBS- nor BMP4-soaked beads were able to induce ectopic expression of YY1 around the site of implantation (A–D). However, BMP4-soaked beads induced a ring of ectopic Msx2 expression around the implanted bead after 24 h (E).