Skip to main content
. Author manuscript; available in PMC: 2023 Apr 24.
Published in final edited form as: Food Chem Toxicol. 2021 Dec 3;159:112722. doi: 10.1016/j.fct.2021.112722

Figure 4. CBD decreases cell cycle-related proteins in TM4 and human Sertoli cells.

Figure 4.

Total cellular proteins were extracted from TM4 and human Sertoli cells after a 24 h CBD treatment. The levels of cyclin D1, cyclin D3, CDK2, CDK4, and CDK6 were determined by Western blotting. GAPDH was used as an internal control. Representative Western blots are shown in A and C, and quantification is presented in B and D. The results represent means ± SD (n=3). The intensity of each protein band was normalized to its individual GAPDH band. #, significant concentration-related linear trend. *, significantly different from the DMSO control.