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(A) First, the 3D model was designed with four different input parameters, and the sample was printed by an extrusion-based bioprinter. (B) Next, the data was acquired using brightfield microscopy. (C) The data were normalized, processed, and interpolated by a Gaussian function. Then the dataset was used as the FIS rules for implementing the FIS model; the final model was generated to approximate and optimize the BPI for the parameter set.
