|
| Antibodies |
|
| AF350 NHS Ester (Live/Dead) |
ThermoFisher |
Catalog # A10168 |
| Rat Anti-Mouse CD16/32 (clone 93) |
Biolegend |
Catalog # 101302 |
| Armenian Hamster anti-Mouse CD11c, PE (clone N418) |
Biolegend |
Catalog # 117308 |
| Rat anti-Mouse CD169, ef660 (clone Ser-4) |
Invitrogen |
Catalog # 50–5755-82 |
| Rat anti-Mouse F4/80, PE (clone BM8) |
Biolegend |
Catalog #123110 |
| Streptavidin, Alexa Fluor 488, Conjugate |
Invitrogen |
Catalog #S11223
|
| Rat anti-Mouse CD45, BUV661 (clone 30-F11) |
BD Bioscience |
Catalog # 565079 |
| Rat anti-Mouse CD8a, BUV737 (clone 53–6.7) |
BD Bioscience |
Catalog # 564297 |
| Rat anti-Mouse Ly6G, PerCP-Cy5.5 (clone 1A8) |
Biolegend |
Catalog # 127616 |
| Rat anti-Mouse CCR2, PE |
R&D Systems |
Catalog # FAB5538P |
| Rat anti-Mouse NK1.1, PE-Cy7 (clone PK136) |
Biolegend |
Catalog # 108714 |
| Rat ant-Mouse CD64, PE-Dazzle 594 (clone X54–5/7.1) |
Biolegend |
Catalog # 139320 |
| Rat anti-Mouse MHCII, Pacific Blue (clone M5/114.15.2) |
Biolegend |
Catalog # 107620 |
| Rat anti-Mouse CD4, Pacific Orange (clone RM4–5) |
Invitrogen |
Catalog # MCD0430 |
| Rat anti-Mouse CX3CR1, BV605 (clone SA011F11) |
Biolegend |
Catalog # 149027 |
| Rat anti-Mouse F4/80, BV650 (clone BM8) |
Biolegend |
Catalog # 123149 |
| Rat anti-Mouse CD11b, BV711 (clone M1/70) |
Biolegend |
Catalog # 101241 |
| Rat anti-Mouse Ly6C, BV786 (clone HK1.4) |
Biolegend |
Catalog # 128041 |
| Rat anti-Mouse B220, AF700 (clone RA3–6B2) |
Biolegend |
Catalog # 103232 |
| Rat anti-Mouse CD11c, APC-eF780 (clone N418) |
Invitrogen |
Catalog # 47–0114-82 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Triton X-100 |
Fisher Scientific |
Catalog # BP151–100 |
| 32% Paraformaldehyde |
EMS Inc |
Catalog # 15714S |
| Sodium Periodate |
Sigma Aldrich |
Catalog #311448 |
| Shandon Immu-Mount |
Fisher Scientific |
Catalog # 9990402 |
| Normal Goat Serum |
Millipore |
Catalog # NS02L |
| Fetal Calf Serum |
Gibco |
Catalog # 16000044 |
| RPMI 1640 with L-Glutamine |
Lonza |
Catalog # BE12–702F |
| DNase I |
Sigma Aldrich |
Catalog # 10104159001 |
| Collagenase IV |
Gibco |
Catalog # 17–104-019 |
| Gibco Pen/Strep |
ThermoFisher |
Catalog # 15140122 |
| CaCl2 |
Fisher Scientific |
Catalog # C79 |
| MgCl2 |
Fisher Scientific |
Catalog # M35 |
| Gentamicin |
Gibco |
Catalog # 15750–060 |
| Disodium Phosphate (Na2HPO4) |
Sigma Aldrich |
Catalog # S3264 |
| Monosodium Phosphate (H2NaO4P) |
Sigma Aldrich |
Catalog # S3139 |
| L-Lysine HCl |
Sigma Aldrich |
Catalog # 4400 |
| Sucrose |
Sigma Aldrich |
Catalog #S8501 |
| IxPBS |
Gibco |
Catalog # 10010023 |
| Endotoxin-Free Ultra Pure Water |
Millipore Sigma |
Catalog # TMS-011-A |
| Endotoxin-Free Dulbecco's PBS (1X) (w/o Ca2+ & Mg++) |
Millipore Sigma |
Catalog #TMS-012-A |
| Phenol LPS - E.coli O111:B4 |
Sigma Aldrich |
Catalog # L2630 |
| LPS-FITC - E.coli O111.B4 |
Sigma Aldrich |
Catalog # F3665 |
| Diphtheria toxin |
Sigma Aldrich |
Catalog # D0564 |
| Recombinant mouse IL-10 |
R&D Systems |
Catalog # 417-ML-025 |
| Anti-mouse Ly6G depleting antibody (1A8) |
BioXCell |
Catalog # BE0075–1 |
| Rat IgG2a isotype control (2A3) |
BioXCell |
Catalog # BE0089 |
| Anti-mouse anti-IL-6 blocking antibody (MP5–20F3) |
BioXCell |
Catalog # BE0046 |
| Rat IgG1 isotype control (HRPN) |
BioXCell |
Catalog # BE0088 |
|
| Critical commercial assays |
|
| Bio-Plex Pro mouse cytokine 23-plex Assay |
Bio-Rad |
Catalog # M60009rdpd |
| Chromo-LAL |
ACCIUSA |
Catalog # C0031–5 |
|
| Experimental models: Organisms/strains |
|
| Mouse: CD169-DTR: B6; 129-Siglec1 < tm1(HBEGF)Mtka> |
New York University: Originally from Makoto Tanaka |
RRID:IMSR_NM-KO-201894 |
| Mouse: C57BL/6-Siglec1 <tm1(cre)Mtka> |
New York University: Originally from Makoto Tanaka |
RRID:IMSR_RBRC06239 |
| Mouse: C57Bl/6NCrl |
New York University: Originally from Charles Rivers |
RRID:IMSR_CRL:027 |
| Mouse: IL-10flox/flox
|
New York University: Originally from Joseph Sun |
RRID:IMSR_NM-CKO-200003 |
| Mouse: B6(cg)-Il10tm1.1Karp/J |
New York University: Originally from The Jackson Laboratory |
RRID:IMSR_JAX:014530 |
|
| Software and algorithms |
|
| FlowJo (Versions 10.7.1) |
Becton Dickinson & Company |
RRID:SCR_008520 |
| Prism (Version 8) |
Graphpad |
RRID:SCR_002798 |
| Illustrator CC2020 |
Adobe |
RRID:SCR_010279 |
| ImageJ - FIJI (Version 2.1.0/1.53c |
NIH |
RRID:SCR_002285 |
| Imaris (Version 9.0) |
Bitplane |
RRID:SCR_007370 |
| Zen Digital Imaging for Light Microscopy, Zen 2012 |
Zeiss |
RRID:SCR_013672 |
| Everest Software (Version 2.3) |
Bio-Rad |
Bio-rad.com
|
| BD FACSDiva (Version 8.01) |
Becton Dickinson & Company |
RRID:SCR_001456 |
