Skip to main content
. Author manuscript; available in PMC: 2023 Apr 24.
Published in final edited form as: J Invest Dermatol. 2022 Sep 27;143(3):456–469.e8. doi: 10.1016/j.jid.2022.07.033

Figure 1. Hypoxia-induced global alterations in glycosylation and glycome-associated genes.

Figure 1.

(a) RNA-sequencing analysis of glycosylation and glycome-associated genes of A375, A2058, and SkMel5 cells cultured under normoxia or hypoxia. (b) HIF1α immunoblot for validation of hypoxia induction in A375 and A2058 cells. Validation of GCNT2 downregulation and Gal-8, KLF4, and NGFR upregulation under hypoxia in (c) A375 and (d) A2058 by RT-qPCR. Flow cytometric analysis of Gal-8 expression on (e) A375 and (f) A2058 cells grown under normoxia or hypoxia. Flow cytometric analysis of i-linear poly-LacNAc expression using OSK-28 antibody on (g) A375 and (h) A2058 cells grown under normoxia or hypoxia. At least four biological replicates were performed. ***P < 0.001 **P < 0.01, and *P < 0.05. Gal-8, galectin 8; GCNT2, β1,6 N-acetylglucosaminyltransferase 2; poly-LacNAc, poly-N-acetyllactosamine.