Figure 6.
RNA-prM/E induced antigen-specific binding and neutralizing antibody responses in pregnant rabbits
Sera collected on weeks 7 and 13 after primary immunization were assayed for (A and B) anti-ZIKV E IgG responses by ELISA and (C) ZIKV (PRVABC56 isolate) neutralization by measuring cytopathic effect (CPE) in Vero E6 cells using the xCELLigence system. (D) Correlation plot between binding and neutralizing responses showing Pearson’s correlation coefficient (r value) and significance of correlation (p value). Mean endpoint titer between RNA-prM/E and RNA-SOSIP-scfl groups was compared using non-parametric Mann-Whitney test (∗∗p < 0.005).