Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 17;11(4):e006334. doi: 10.1136/jitc-2022-006334

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Validation of the Co-HA system. (A) Construction of the KRAS G12D TCR-T cells from healthy donors detected by FCM. (B) The specific proliferation levels of the KRAS G12D TCR-T cells detected by FCM after co-culture with the COS-7 cells expressing the relevant neoantigen in the Co-HA system. (C) The specific secretion of IFN-γ by TCR-T cells against the KRAS G12D neoantigen in the Co-HA system detected by ELISpot. (D) The specific secretion of IFN-γ by TCR-T cells against the KRAS G12D neoantigen in the Co-HA system detected by ELISA. (E) The specific lysis of the COS-7 cells generated by the KRAS G12D neoantigen in the Co-HA system detected by Sanger sequencing. (F) The LDH levels induced by TCR-T cells against the KRAS G12D neoantigen in the Co-HA system detected by ELISA. FCM, flow cytometry; ELISpot, enzyme-linked immunospot assay; IFN-γ, interferon-γ; LDH, lactate dehydrogenase; TCR, T-cell receptor.