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Interaction of HDAC10 with HDAC3. Flag-tagged HDAC10 (lanes 1, 4, 6 and 9) or the mutant H135A (lanes 2, 5, 7 and 10) were expressed with (lanes 4 and 5) or without (lanes 1–2) HA-HDAC3 in 293 cells and immunoprecipitated with anti-Flag M2 agarose. Extracts (lanes 1–5) and immunoprecipitated proteins (lanes 6–10), which were eluted with Flag peptide, were subjected to western blotting analysis with anti-Flag (A) or anti-HA (B) antibody.
