Table 1.

Detection of Mayaro virus using RT-qPCR in pools of female Mansonia humeralis

RT-qPCR MAYV			RT-qPCR MAYV
Pool number	Cq	Number of copies/µl	Pool number	Cq	Number of copies/µl
2	34.47	10	25	33.82	16
3	33.45	19	26	33.61	18
4	31.59	61	28	27.39	821
5	33.05	25	30	30.74	104
6	32.90	27	31b	28.69	368
8	29.30	253	32	33.43	20
10	32.69	31	34	33.40	20
12	31.58	62	41	31.19	79
14	30.45	124	42	31.24	76
15	33.92	15	43c	29.14	279
18	34.71	9	45	34.74	9
19	34.53	10	46d	28.88	327
20	30.90	94	48	25.73	2287
21	32.12	44	49	34.41	11
22a	26.27	1639	50	30.71	106
23	30.26	140	57	34.58	10
24	33.02	25	184	26.82	1167

^a–dThese pools were used for the in vitro infection experiment