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. 2023 Mar 11;299(4):104601. doi: 10.1016/j.jbc.2023.104601

Figure 3.

Figure 3

Structural basis for the recognition of H3G34R.A, the crystal structure of the RACK7 PHD finger in complex with H3G34R peptide (PDB ID: 7CWH). Electrostatic surface potential of the PHD finger is shown with blue and red colors representing surface positive and negative charges, respectively. The H3G34R peptide is depicted as a cyan ribbon, with R34 shown as sticks and labeled. B, overlay of the crystal structures of the RACK7 PHD finger (wheat) in complex with H3G34R peptide (cyan) (PDB ID: 7CWH) and the DIDO PHD finger (gray) in complex with H3K4me3 peptide (dark pink) (PDB ID 4L7X). Histone peptides are depicted as ribbons. C, structural overlay of the RACK7 PHD finger (wheat) in complex with H3G34R peptide (cyan) (PDB ID: 7CWH) and the RACK7 PHD-BD-PWWP cassette (gray) (PDB ID 5B73). The H3G34R peptide residues and the PHD finger residues involved in the interaction with the peptide are shown as sticks and labeled. Intermolecular hydrogen bonds are indicated by yellow dash lines. The zinc ions are gray spheres. D, structural overlay of the RACK7 PHD finger (wheat) in complex with H3G34R peptide (cyan) (PDB ID: 7CWH), the RACK7 PHD-BD-PWWP cassette (gray) (PDB ID 5B73), and the unmodified H3 (green)-bound BHC80 PHD finger (gray) (PDB ID 2PUY). H3G34R peptide and unmodified H3 peptide are shown as ribbons, and R34 and V35 of the oncohistone are labeled. E, structural overlay of the RACK7 PHD finger (wheat) in complex with H3G34R peptide (cyan) (PDB ID: 7CWH), the RACK7 PHD-BD-PWWP cassette (gray) (PDB ID 5B73), and the ZMYND11 BD-PWWP cassette in complex with H3K36me3 peptide (dark blue) (PDB ID 4N4H). PHD, plant homeodomain.