Figure 10.

P132L localization defect is rescued by co-expression of WT CAV1 in CAV1^−/−MEFs. Representative AiryScan images are shown for CAV1^−/− MEF cells expressing (A) CAV1-mCherry, (B) P132L-myc, (C) CAV1-mCherry plus P132L-myc, (D) CAV1-mCherry plus CAV1-myc, or (E) CAV1-myc. Cells were allowed to express the indicated constructs for 24 h, fixed, and immunostained for endogenous cavin and myc-tagged constructs prior to imaging. Bar represents 5 μm. In (A–E), red arrowheads point to examples of colocalized puncta. F–H, Pearson’s correlation analysis (n = 90 ROIs from three independent experiments). A one-way ANOVA with Tukey's test (≥3 groups) or an unpaired Student's t test (2 groups) was used to calculate p-values. n.s., not significant.