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P132L forms mixed 8S oligomers with WT CAV1 in Escherichia coli cells as assessed by BN-PAGE. Total membrane from E. coli cells expressing the indicated constructs was lysed in C12M and subjected to BN-PAGE followed by Western blotting for mVenus (GFP, green) and CAV1 (red). Lane numbers were labeled at the bottom. The range of high molecular bands is indicated with red brackets. CV, CAV1-mVenus; PV, P132L-mVenus.
