Skip to main content
. 2023 Mar 3;299(4):104574. doi: 10.1016/j.jbc.2023.104574

Figure 7.

Figure 7

P132L forms mixed 8S complexes with WT CAV1 as assessed by negative stain EM. Purified CAV1 complexes were negatively stained and visualized by EM. Representative images of negatively stained P1 fractions of (A) CAV1-mVenus, (B) CAV1, (C) P132L-Venus, (D) P132L, (E) CAV1 + CAV1-mVenus, (F) P132L + P132L-mVenus, (G) CAV1 + P132L-mVenus, and (H) P132L + CAV1-mVenus. Scale bar represents 30 nm. Representative 2D averages of (I) CAV1 complexes, (J) CAV1-mVenus 8S complexes, (K) CAV1 + CAV1-mVenus mixed complexes, and (L) CAV1 + P132L-mVenus mixed complexes. The arrow marks regions of additional density from the Venus tag. Scale bars represent 10 nm.