Table 1.
Effect of P132L mutation on the predicted stability of adjacent residues
| Residue | Rosetta ddG | ConSurf score |
|---|---|---|
| W115i+1 | 0.51 | 5 |
| W128i | −0.09 | 9 |
| A129i | 0.10 | 4 |
| V130i | 0.55 | 6 |
| V131i | 0.41 | 6 |
| P132i | −0.21 | 9 |
| C133i | 0.10 | 5 |
| I134i | 0.01 | 5 |
| K135i | −0.05 | 6 |
| S136i | 0.22 | 5 |
| V71i+2 | 0.81 | 8 |
| I72i+2 | 0.74 | 8 |
Per-residue Rosetta energies of 12 amino acids within 8 Å of P132L were subtracted from that of the P132P mutation. Residues predicted to be most strongly impacted by the P132L mutation are highlighted in bold. ConSurf scores (84) are provided for each residue (range of 1–9, where 1 = variable, 5 = average, and 9 = highly conserved). See Figures 1 and 2 for the positioning of protomers i, i+1, and i+2 in the complex. All ddG units are in Rosetta Energy Units (REU).