Table 4. Thermal stability of DNA hairpin oligonucleotide following incorporation of m¹A at position A3, A7, A8 or A10 and mass spectrometric results, taking into account that m¹A is a ribonucleoside.

Sequence	Tm
5′-d(GGACGTAATAGTCC)	55.5°C
5′-d(GGm1ACGTAATAGTCC)	n.d.
5′-d(GGACGTm1AATAGTCC)	58.5°C
5′-d(GGACGTAm1ATAGTCC)	56.5°C
5′-d(GCACGTAATm1AGTCC)	51.0°C

n.d., no melting point detected.