Skip to main content
. 2023 Apr 24;12:e83545. doi: 10.7554/eLife.83545

Figure 7. Protein synthesis labeling via dietary phenylacetyl-OPP (PhAc-OPP) administration.

Figure 7.

(A) Subtle detection of protein synthesis labeling by AF488-azide following exposure of pan-neuronal penicillin G acylase (PGA)-expressing flies to dietary PhAc-OPP (4 mM) for 48 hr. Brains were counterstained with Dylight Phalloidin-650. (B) AF488-azide quantitation after varying concentrations of PhAc-OPP exposure in pan-neuronal PGA-expressing flies. Significant effect of PhAc-OPP concentration on labeling (ANOVA, Bonferroni post-test, ***p<0.001, ****p<0.0001, n=12–17 brains per group). (C) No significant effect of PhAc-OPP (4 mM) on food intake levels over 4 hr (ANOVA, Bonferroni post-test, ns, n=4 groups of eight flies per condition).