Table 3.
Differentially gene expression and pathway analysis using LPAEx as the comparator group on liver
| Group Compared with LPAEx | Total DEGs | Upregulated | Downregulated | Top Three Genes Up/Downregulated | Top Relevant BioPlanet 2019 Pathways | FDR |
|---|---|---|---|---|---|---|
| LPASed | 112 | 105 | 7 | All DEGs | ||
| Glycoprotein VI-mediated activation cascade | <0.001 | |||||
| Leukocyte transendothelial migration | 0.007 | |||||
| Natural killer cell-mediated cytotoxicity | 0.007 | |||||
| ENSRNOG000000 | Upregulated DEGs | |||||
| 37549 | Glycoprotein VI-mediated activation cascade | 0.001 | ||||
| A1bg | Leukocyte transendothelial migration | 0.010 | ||||
| Ank1 | Interleukin-4 regulation of apoptosis | 0.010 | ||||
| Ube2b | Downregulated DEGs | |||||
| ENSRNOG000000 | N/A | |||||
| 48864 | ||||||
| Nr1d1 | ||||||
| LPEx | 19 | 11 | 8 | All DEGs | ||
| Cholesterol biosynthesis | <0.001 | |||||
| Lipid and lipoprotein metabolism | <0.001 | |||||
| Ank1 | Upregulated DEGs | N/A | ||||
| Rrm2 | ||||||
| Hba-a3 | ||||||
| Abcg8 | Downregulated DEGs | |||||
| Abcg5 | N/A | |||||
| Tlcd2 | ||||||
| LPSed | 143 | 137 | 6 | All DEGs | ||
| Interleukin-2 signaling | <0.001 | |||||
| Endogenous Toll-like receptor signaling | <0.001 | |||||
| A1bg | Upregulated DEGs | |||||
| ENSRNOG000000 | Interleukin-2 signaling | <0.001 | ||||
| 09329 | Endogenous Toll-like receptor signaling | <0.001 | ||||
| Ank1 | ||||||
| Nr1d1 | Downregulated DEGs | |||||
| Hsd17b2 | ||||||
| Dbp | ||||||
| CONEx | 258 | 252 | 6 | All DEGs | ||
| Interleukin-2 signaling | <0.001 | |||||
| Interleukin regulation of apoptosis T cell receptor regulation of apoptosis |
<0.001, <0.001 |
|||||
| A2m | Upregulated DEGs | |||||
| Ank1 | Interleukin-2 signaling | <0.001 | ||||
| Faahl | Interleukin regulation of apoptosis | <0.001 | ||||
| T cell receptor regulation of apoptosis | <0.001 | |||||
| ENSRNOG000000 | Downregulated DEGs | |||||
| 04419 | N/A | |||||
| Zswim8 | ||||||
| Scrn1 | ||||||
| CONSed | 73 | 63 | 10 | All DEGs | ||
| Circadian rhythm | 0.023 | |||||
| Interleukin-7 signaling pathway | 0.028 | |||||
| A1bg | Upregulated DEGs | |||||
| Ank1 | Interleukin-4 regulation of apoptosis | 0.030 | ||||
| Rrm2 | Interleukin-7 signaling pathway | 0.030 | ||||
| Ube2b | Downregulated DEGs | |||||
| ENSRNOG00000048864 | N/A | |||||
| Dbp |
The pathway analysis were performed using Bioplanet2019 as the reference database in all comparisons with >15 genes differently expressed. Pathways with a FDR < 0.10 were considered statistically significant. CONEx, control + exercise group; CONSed, control + sedentary group; DEG, differential expressed genes; FDR, false discovery rate; LPEx, Lactobacillus paracasei + exercise group; LPSed, Lactobacillus paracasei + sedentary group; LPAEx, Lactobacillus paracasei expressing angiotensin (1–7) + exercise group; LPASed, Lactobacillus paracasei + sedentary group; N/A, not applicable.