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. Author manuscript; available in PMC: 2023 Apr 24.
Published in final edited form as: Nat Chem Biol. 2022 Feb 24;18(4):412–421. doi: 10.1038/s41589-022-00971-2

Extended Data Figure 3. Structure-activity relationships of EN523 analogs with OTUB1.

Extended Data Figure 3.

Gel-based ABPP analysis EN523 analogs against OTUB1. Vehicle DMSO or EN523 analogs were pre-incubated with recombinant OTUB1 for 30 min at 37 °C prior to IA-rhodamine labeling (100 nM, 30 min room temperature). OTUB1 was then separated by SDS/PAGE and in-gel fluorescence was assessed. Also shown is silver staining showing protein loading. Shown are representative gels of n=3 biologically independent samples/group.