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. 2023 Feb 15;3(2):158–173. doi: 10.1021/acsbiomedchemau.2c00067

Scheme 1. Synthetic Scheme for the Peptides by Using Rink Amide Resin Exemplified by Peptide NT-02.

Scheme 1

Reagents and conditions: (a) 20% piperidine in DMF, 3 min (two times); (b) Fmoc-d-Ala-OH (10 equiv), HBTU (10 equiv), DIPEA (10 equiv), DMF, 7 min; (c) Fmoc-d-Phe-OH (5 equiv), HBTU (5 equiv), DIPEA (10 equiv), DMF, 7 min; (d) Fmoc-d-Leu-OH (5 equiv), HBTU (5 equiv), DIPEA (10 equiv), DMF, 7 min; (e) Fmoc-d-Lys (Boc)-OH (5 equiv), HBTU (5 equiv), DIPEA (10 equiv), DMF, 7 min; (f) 92.5% TFA, 2.5% water, 2.5% phenol, 2.5% 1.2-ethylenedithiol, 12 h.