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. 2002 Mar 1;30(5):e19. doi: 10.1093/nar/30.5.e19

Figure 2.

Figure 2

(Left) CFET tag-labeled oligonucleotides. The CFET tags are constructed from three chromophores FAM (F), TAM (T) and ALEXA 594 (A). The number of 1′,2′-dideoxyribose phosphate spacers (S) between the chromophores is indicated in the CFET tag designation: FAM (CFET-1); T-4-T (CFET-2, m = 4); F-9-T (CFET-3, m = 9); F-13-T (CFET-4, m = 13); F-5-A (CFET-5, m = 5, n = 13); F-7-A (CFET-6, m = 7, n = 10); F-10-A (CFET-7, m = 10, n = 11); F-13-A (CFET-8, m = 13, n = 8). Spacers (S)m,n are used to separate the chromophores to tune the ET efficiency and as mobility tags for electrophoresis analysis. The CFET-labeled oligonucleotides (20 bp) are designed to be complementary to the DNA template containing multiple SNPs. (Right) A synthetic scheme for the preparation of a CFET tag containing FAM and ALEXA.