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. 2002 Mar 1;30(5):e19. doi: 10.1093/nar/30.5.e19

Figure 3.

Figure 3

SBEs for multiplex SNP detection using CFET-labeled primers and biotin-ddNTPs. DNA template containing polymorphic sites is incubated with CFET-labeled primers, biotin-ddNTPs and DNA polymerase. The nucleotide at the 3′-end of each CFET-labeled primer is complementary to a particular SNP in the template. Only the fully complementary CFET-labeled primer was extended by DNA polymerase with a biotin-ddNTP. After solid phase capture and isolation of the biotinylated DNA extension fragments the SBE products are analyzed for their fluorescence signatures, each of which codes for a unique SNP.