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Electropheretogram of CFET-labeled SBE products for SNP identification in exon 20 of the RB1 gene. (A) Detection of six nucleotide variations from synthetic DNA templates. The FAM and T-4-T peaks detected a heterozygous genotype (T/A), while the F-9-T and F-13-T peaks identified a C/T heterozygous genotype. The F-5-A and F-10-A peaks identified another heterozygous genotype (G/T). (B) Detection of three homozygous genotypes (T, C and G) for a PCR product from the RB1 gene.
