Figure 2.

Curcumin-reduced pyroptosis induced by a high-glucose environment. Glu: glucose; Cur: curcumin; the dose of curcumin used in cell experiments is 14 μM. (a) The expression levels of related proteins in the pyroptotic pathway were detected by western blotting. (b) The expression level of NLRP3 in cardiomyocytes was detected by immunofluorescence (40x). (c) The levels of reactive oxygen species (ROS) in cardiomyocytes of each group were detected by ROS fluorescent probing (20x). (d) The apoptosis level of cardiomyocytes in each group was detected by TUNEL fluorescent probing (20x). ^∗∗P < 0.001, high-glucose group vs. normal group and curcumin-treated group. Values are expressed as the mean ± standard deviation.