Figure 6.

ml385 inhibits flavin protective effects in diabetic myocardial injury. DM: diabetic rat; Cur: curcumin; the dose used in this experiment was 200 mg/kg/day. ml385: inhibitor of the Nrf2 pathway, the dose used in the experiment was 20 mg/kg. (a) PCR and western blotting to verify the inhibitory effect of ml385 on the Nrf2 pathway. (b) Detection of protein expression of the AKT/Nrf2 and pyroptosis pathways in cardiac tissue by western blotting. (c) Myocardial cross-section and the ratio of the heart weight to body weight (HW/BW). (d) Immunohistochemical results of NLRP3 and caspase-1 staining in rat myocardial tissue (20x). (e) TUNEL was used to detect the apoptosis rate of myocardial cells of rats in each group (20x). (f) Hematoxylin and eosin (HE) staining, Masson's staining, and collagen II fluorescence detection of myocardial tissue (20x). ^∗∗P < 0.001: diabetic rats vs. control and curcumin-treated groups. #P < 0.05: injected with ml385 vs. not injected (in diabetic rats). #^∗P < 0.001: injected with ml385 vs. not injected (in diabetic rats). Values are expressed as the mean ± standard deviation.