Key resources table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Bacterial and virus strains
Biological samples
Chemicals, peptides, and recombinant proteins
Critical commercial assays
Deposited data
Experimental models: Cell lines
Experimental models: Organisms/strains
yw; ParB2-eGFP; eNosx2-MCP-mCherry; +
yw; Dorsal-mVenus, pNos-MCP-mCherry; pNos-MCP-mCherry, His2Av-iRFP
yw; Dorsal-mVenus, pNos-MCP-mCherry; Dorsal-mVenus, pNos-MCP-mCherry, His2Av-iRFP
yw; dl1, pNos-MCP-mCherry; pNos-MCP-mCherry, His2Av-iRFP
yw; 1Dg(11) ; +		DBS_6.23
yw; 1DgS(2) ; +		DBS_5.81
yw; 1DgW(2) ; +		DBS_5.39
yw; 1DgAW(3) ; +		DBS_5.13
yw; 1DgSVW(2) ; +		DBS_4.8
yw; 1DgVW(1) ; +		DBS_4.73
yw; 1DgVVW(3) ; +		DBS_4.29
yw; 2xIntB2–1Dg(4) ; +
Oligonucleotides
5’ ggaacgaaggcagttagttgt		18.8
5’tagttccagtgaaatccaagcattttc		Ori-Seq-F1
5’ ccattaaaacggaaccaagaggtgag		OutLHA
5’ tctaacaatggctcgatttttgcca		OutDlRHA
Recombinant DNA
pIB-1Dg-evePr-MS2v5-LacZ-Tub3UTR		DBS_6.23
pIB-1DgS-MS2v5-LacZ-Tub3UTR		DBS_5.81
pIB-1DgW-MS2v5-LacZ-Tub3UTR		DBS_5.39
pIB-1DgAW-MS2v5-LacZ-Tub3UTR		DBS_5.13
pIB-1DgSVW-MS2v5-LacZ-Tub3UTR		DBS_4.8
pIB-1DgVW-MS2v5-LacZ-Tub3UTR		DBS_4.73
pIB-1DgVVW-MS2v5-LacZ-Tub3UTR		DBS_4.29
pIB-4xIntB2-Neutral400–1Dg-MS2v5-LacZ-Tub3UTR
Dl-mVenus-dsRed
pU6-DlgRNA1
pBPhi-eNosx2-pTrans-NoNLS-MCP-mCherry-tub3’UTR
pCasper4-His2Av-iRFP
pCasper4-Pnos-NoNLS-MCP-mCherry-TUB3’UTR
pCasper-pNos-NoNLS-ParB2-GFP-TUB3’UTR
Software and algorithms
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/
Matlab
Patser	Hertz GZ and Stormo GD, 1999.
Image analysis pipeline	Garcia HG, Tikhonov M, Lin A, Gregor T. Quantitative Imaging of Transcription in Living Drosophila Embryos842 Links Polymerase Activity to Patterning. Current Biology. 2013 Nov; 23(21):2140–2145 Lammers NC, Galstyan V, Reimer A, Medin SA, Wiggins CH, Garcia HG. Multimodal transcriptional control902 of pattern formation in embryonic development. PNAS. 2020 Jan; 117(2):836–847.	https://github.com/GarciaLab/mRNADynamics
Post image processing data analysis pipeline	This paper	https://github.com/GarciaLab/mRNADynamics_dorsal_synthetics/releases/tag/v1.0.0
MCMCSTAT pipeline	H. Haario, M. Laine, A. Mira and E. Saksman, 2006. DRAM: Efficient adaptive MCMC, Statistics and Computing 16, pp. 339–354. doi: 10.1007/s11222-006-9438-0	https://github.com/mjlaine/mcmcstat
Other