Fig. 7. Toxicology of DL-1 and DL-9 in primary human hepatocytes.

a–e Micropatterned co-cultures of primary human hepatocytes were treated DL-1 or DL-9 for up to 13 days at concentrations ranging from 0.75 to 48 µg/ml. Rifampin (25 µM) and omeprazole (50 µM) treated cells were included as controls. a Micrographs showing the impact of DL-1 and DL-9 on cell morphology after treatment for 8, 10, and 12 days with 48 µg/ml of each compound. b–e Bar graphs showing the level of Albumin (b, c) and Urea (d, e) in the medium of hepatocytes treated with 0.75, 3, 6, 12, 24, and 48 µg/ml of DL-1 or DL-9 for 9, 11, or 13 days (b, c) or 5, 7, 9, 11, and 13 days (d, e). Data presented as mean ± SD of three technical replicates (n = 3) and statistical analysis was performed using two-way ANOVA.