Table 2.
Flow cytometry and cell sorting antibodies
| Target with conjugate | Dilution | Company | Cat. No. |
|---|---|---|---|
| B220-APC | 1:100 | BioLegend | 103211 |
| CD11b-BUV805 | 1:800 | BD Biosciences | 741934 |
| CD11b-PE-Cy7 | 1:800 | BD Biosciences | 552850 |
| CD19-Alexa700 | 1:50 | BD Biosciences | 557958 |
| CD25-PE | 1:200 | Life Technologies | 12-0251-82 |
| CD31-APC | 1:100 | BD Biosciences | 561814 |
| CD3-PerCp-Cy5.5 | 1:50 | BioLegend | 100217 |
| CD45-PerCp-Cy5.5 | 1:200 | BD Biosciences | 550994 |
| CD45-FITC | 1:400 | BD Biosciences | 553079 |
| CD4-PE-Cy7 | 1:300 | Life Technologies | 25-0041-82 |
| CD8-APC-Cy7 | 1:100 | BD Biosciences | 557654 |
| DAPI (stock: 10,5 mM) | 1:10.000 | Life Technologies | D3571 |
| F4/80-Alexa Fluor 488 | 1:100 | Life Technologies | 53-4801-82 |
| FoxP3-Alexa Fluor 488 | 1:50 | BD Biosciences | 560403 |
| Ly6C-PE | 1:200 | BD Biosciences | 560592 |
| Ly6G-APC-Cy7 | 1:100 | BD Biosciences | 560600 |
| MHC-II-BV510 | 1:100 | BD Biosciences | 742893 |
| PDPN-APC-Cy7 | 1:100 | BioLegend | 127417 |
| SiglecF-BUV395 | 1:100 | BD Biosciences | 740280 |