Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 24;13:6620. doi: 10.1038/s41598-023-33698-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Verification of URT-PCR sensitivity. The relative sensitivity of the URT-PCR strategy was tested against the commercial standard (probe-based qRT-PCR (TaqMan). For six of ten direct comparisons, URT-PCR detected trqnscript, but TaqMan did not; examples (Panel A) miR-140 and (Panel B) miR-423-3p are shown. (Top) Total RNA (1 ng/ul) from a mixture of epithelial lung cells pooled was interrogated by both methods; URT-qPCR (left) showed CT 27 versus 31 for TaqMan, or 2^4 = 16-fold more sensitive. (Bottom) Total RNA extracted from EBC by conventional means (Methods) showed miR 423-3p as detectable by URT-qPCR, but absent by TaqMan).