Figure 6.

Possible factors contributing to reduced EGFR expression in thyroid cultured cells. (A) Expression of EGFR was tested by Real time PCR in five different FND samples. Cells treated with miR-7-5p inhibitor and miR-146b-5p mimic show significant downregulation of EGFR (**p=0.005). Oxidative stress combined with miR-7-5p downregulation and miR-146b-5p upregulation resulted in the highest reduction of EGFR expression (***p < 0.001). (B) EGFR protein expression in the same conditions was tested by western blot and showed similar results.