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. 2023 Apr 11;13:1129627. doi: 10.3389/fonc.2023.1129627

Figure 1.

Figure 1

Histological and biological characteristics of GBM PDX. (A) Analysis of three representative patient tissue samples and respective s.c. PDX tissue from in vivo passage #2 revealed comparable histology (HE), MGMT expression (red staining) and expression of proliferation marker Ki-67 (brown staining), 200-fold magnification, inset 800-fold magnification. (B) Comparable growth over several consecutive s.c. passages of PDX models Glio11368 and Glio12464. (C) Heterogeneous tumor doubling times in our panel of established PDX models, n=3-6. Mean and standard deviation (SD). (D) Comparison of nodular and infiltrative growth in two different orthotopic (intracerebral, i.cer.) PDX models (cresyl violet staining, tumor tissue stained purple) (0.9-fold magnification). (E) Analysis of PDX Glio12464 revealed comparable histology and Ki-67 expression over several consecutive s.c. passages and parallel orthotopic inoculation. Examination of Ki-67 expression (proliferation marker) in PDX tumor tissue via IHC. Positive areas in the sections are stained brown. 20-fold magnification, inset 160-fold magnification.