FIGURE 2.

Screening and data analysis workflow. Eggs were collected after 1 h of pair- or group-wise mating before being incubated overnight. At 21 h post-fertilization (hpf), embryos were dechorionated, then at 24 hpf were treated with compounds from the Prestwick drug library. Treatment was stopped at 48 hpf, and fish were mounted in orientation plates to visualize fluorescent hearts and imaged with an ACQUIFER imaging machine. Displayed data types can be quantified using the pyHeart4Fish GUI and result in a graphical output in the form of GIFs and PNGs, as well as data sheets with additional meta and quantitative data. This figure was created with BioRender.com.