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. 2023 Apr 11;14:1126119. doi: 10.3389/fphar.2023.1126119

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Copyright © 2023 Otkur, Liu, Chen, Li, Ling, Lin, Yang, Xia, Qi and Piao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CID-2745687 attenuated anchorage-independent cell growth in GPR35-expressing CRC cells. (A,B) Representative images of colonies formed by HCT116 cell lines in soft agar in 21 days and quantification data of soft agar colony formation assay (n = 3). Scale bars: 0.5 cm. (C,D) Representative images of colonies formed by DLD1 cell lines in soft agar in 2 days, and quantification data of soft agar colony formation assay (n = 3). Scale bars: 0.5 cm. (E,F) Representative images of colonies formed by LS174T cell lines in soft agar in 21 days, and quantification data of soft agar colony formation assay (n = 3). Scale bars: 0.5 cm. Cells were suspended in a medium with 10 μM CID and seeded in soft agar. The medium was refreshed, and 10 μM of CID was added every 3 days.