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. 2023 Apr 11;14:1126119. doi: 10.3389/fphar.2023.1126119

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Copyright © 2023 Otkur, Liu, Chen, Li, Ling, Lin, Yang, Xia, Qi and Piao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GPR35 expression moderated YAP/TAZ phosphorylation. (A) Western blot assay on YAP, TAZ, phosphorylated YAP, TAZ, and LATS1 after the indicated treatments. 293FT cells harvest for the assay after 48 h of incubation with different concentrations of GPR35 expression lentivirus. (B) Western blot analysis of phosphorylated YAP. After 24 h of culture, cells were subject to fresh medium containing 10% FBS. Cells were harvested at indicated time points; (C) Western blot assay on phosphorylated YAP after serum deprivation. After 24 h of culture, the medium was replaced by fresh serum-free medium and incubated for the indicated time.