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. 2023 Apr 11;14:1126119. doi: 10.3389/fphar.2023.1126119

FIGURE 5.

FIGURE 5

CID-2745687 inhibited GPR35-meditated YAP/TAZ activity. (A,B) Western blot assay on TAZ expression and YAP phosphorylation in established cell lines were challenged with serum deprivation for 1 h. Cells were subjected to fresh serum-free medium, and different CID concentrations were added simultaneously with serum-free medium. (C) Western blot analyzing expression and phosphorylation of YAP, TAZ, and LATS1 after treatment with different concentrations of CID. 293FT cells were infected with GPR35 expression lentivirus at the ratio of 1:10 for 24 h, and the different concentrations of antagonists were added directly to the medium. (D) mRNA expression of YAP/TAZ target genes after treatment of CID. (E) mRNA expression of YAP/TAZ target genes. 293FT cells were infected with different volumes of GPR35 expression lentivirus for 24 h, and CID was added directly to the medium for another 24 h. Then cells were harvested for PCR assay.