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. 2023 Apr 11;14:1126119. doi: 10.3389/fphar.2023.1126119

FIGURE 8.

FIGURE 8

GPR35 agonists did not promote YAP/TAZ activity but attenuated the regulatory effect of CID-2745687. 293FT cells were infected with GPR35 expression lentivirus at different ratios of 1:20 for 24 h, and 50 μM zaprinast or 20 μM pamoic acid were added directly to the medium. (A) mRNA expression of YAP/TAZ target genes after treatment of 50 μM zaprinast or 20 μM pamoic acid. (B) mRNA expression of YAP/TAZ target genes after treatment of pamoic acid combined with different concentrations of CID. (C) Western blot analysis of YAP phosphorylation and TAZ expression. 293FT cells were infected with GPR35 expression lentivirus at different ratios of 1:10 for 24 h, and indicated concentrations of agonists were added directly to the medium. After 1 h of incubation, different concentrations of CID were added. Cells were harvested for analysis after 24 h of drug treatment. (D) mRNA expression of CTGF, LS174T cells were exposed to indicated treatments for 12 h, and harvested for analysis. (E) mRNA expression of CTGF and CYR61 in LS174T cells after 12 h of agonists treatments with or without 20 μM of CID.