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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Oral Oncol. 2023 Feb 9;138:106330. doi: 10.1016/j.oraloncology.2023.106330

Figure 1. Generation of PDO.

Figure 1.

(A) Schematic showing workflow for generation of PDO. Freshly tumor biopsies are dissociated to single cells, suspended in Matrigel, and plated. After 7–11 days, single cell-derived organoids are formed and may be utilized for further experiments or characterization. (B) 20X hematoxylin and eosin (H&E) image of a representative HNSCC PDO showing a multicellular structure with layers of squamous epithelial cells. Scale bar represents 100 μm.