Reagent	Final concentration	Amount
Advanced DMEM/F-12	n/a	500 mL
HEPES (1 M)	10 mM	5 mL
GlutaMAX (200 mM) Penicillin-Streptomycin (10,000 U/mL)	2 mM 100 U/mL	5 mL 5 mL
Total	n/a	515 mL