Reagent	Final concentration	Amount
Basal medium	n/a	Top up to 100 mL
Dexamethasone (10 μM) 8-Bromo-cAMP (100 mM) IBMX (50 mM) B-27 supplement (50×) Primocin (50 mg/mL) WNT3A* CHIR99021* (10 mM)	50 nM 100 μM 100 μM 1× 100 μg/mL 50% 3 μM	500 μL 100 μL 200 μL 2 mL 200 μL 50 mL 30 μL
Total	n/a	100 mL