Table 1.
Description of the different supplementation strategies studied at lab-scale in shake flasks with the corresponding DuckCelt®-T17 growth kinetic parameters. For each strategy, the supplemented basic culture medium is the OptiPRO™ SFM medium with antibiotics and Pluronic F68
| Strategy | Description | µmax (h− 1) | tD (h) | IVCCa (106 cells.d/mL) |
|---|---|---|---|---|
| A | Glutamine (4 mM, D0) | 0.023 | 30.7 | 26.1 ± 1.1 |
| B | Glutamax (4 mM, D0) | 0.019 | 35.7 | 30.0 ± 1.5 (*) |
| C | Without glutamine | – | – | 3.5 ± 0.2 |
| D | Glutamine (4 mM, D0) + Glutamax (4 mM, D0) | 0.016 | 43.1 | 23.0 ± 1.1 |
| E | Glutamax (4 mM, D0) + Glutamax (4 mM, D0) | 0.019 | 35.9 | 29.6 ± 1.1 (*) |
| F | Glutamine (4 mM, D0) + Glucose (D3, D6) | 0.019 | 36.1 | 29.8 ± 0.7 (*) |
| G | Glutamax (D0) + Glucose (D3, D6) | 0.018 | 39.2 | 27.8 ± 0.7 |
| H | Glutamine (4 mM, D0) + Glutamax (4 mM, D3) | 0.018 | 39.4 | 26.6 ± 0.7 |
| I | Glutamine (2mM, D0) + Glutamine (2mM, D3) | 0.019 | 37.3 | 31.4 ± 2.2 (*) |
| J | Glutamax (2mM, D0) + Glutamax (2mM, D3) | 0.019 | 37.3 | 31.0 ± 1.7 (*) |
| K | Glutamine (4 mM, D0) + SFM (D3, D6, D9) | 0.019 | 35.9 | 31.5 ± 0.4 (*) |
| L | Glutamine (4 mM, D0) + SFM w/o Gln (D3, D6, D9) | 0.019 | 35.4 | 29.0 ± 0.6 (*) |
| M | Glutamax (4 mM, D0) + SFM (D3, D6, D9) | 0.016 | 43.9 | 27.0 ± 0.7 |
a Results are presented as mean ± SEM (n = 6), asterisk (*) indicates a p-value < 0.05 in t-test analysis