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. 2023 Apr 25;14:2379. doi: 10.1038/s41467-023-37865-3

Fig. 2. The central LCD segment of NCAP demonstrates amyloid-like characteristics.

Fig. 2

a X-ray diffractions of LCD-only fibrils (No RNA), and LCD fibrils grown with hairpin-Site2 (S2hp) vRNA or non-specific RNA (antisense siDGCR8-1), show amyloid-characteristic 4.7 and 10 Å reflections labeled by white arrows. b Negative stain EM micrographs of LCD fibrils grown in the presence of the short vRNA segments Site1 (S1; 11 nucleotides), Site 1.5 (S1.5; 22 nucleotides) and Site2 (S2; 22 nucleotides), as well as with a non-specific RNA (antisense siDGCR8-1). All RNA sequences are given in Supplementary Table 1. This figure shows that the central LCD produces amyloid-typical fibrils in the absence and presence of different RNA segments and that the RNA sequence may influence the morphology of the LCD assemblies over time.