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. 2023 Feb 22;18(4):734–745. doi: 10.1021/acschembio.3c00035

Figure 4.

Figure 4

Selectivity of compounds 6e, 7, 11a, and 11b against activities of (A) CamA, (B) CcrM, (C) Dam, (D) MettL3–MettL14, (E) MettL5–Trm112, (F) MettL16, and (G) PCIF1. The oligonucleotide substrates and reaction conditions are listed in Table S2. Compound 7 used in the selectivity experiments was from ref (49). (H) Hydrophobic patch in CamA. The sequence logo shows that the eight residues forming this surface patch are very highly conserved among 58 nonidentical CamA orthologs, but they are not conserved among other DNA/RNA adenine MTases. The catalytic motif IV (D/N)PP(Y/F/W) is highlighted in yellow, the relevant P within the motif in green, and the other patch residues in cyan. In the non-CamA MTases, aligned different (but still hydrophobic) residues are shown in gray, and charged residues are shown in red circles.