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. 2023 Apr 3;12(4):1081–1093. doi: 10.1021/acssynbio.2c00587

Figure 6.

Figure 6

Fratricide REULR and WNT signaling potentiation. (A) Schematic representation of a RNF43- or ZNRF3-based fratricide REULR in the context of a canonical WNT signaling pathway. (B) HEK293STF cells were seeded at 10k/well and subsequently treated with RNF43 or ZNRF3 fratricide REULR for 24 h. FZD cell surface levels were measured by incubating cells with a biotinylated pan-FZD Darpin (DRPB_Fz7/8), recovered by SA647 and analyzed by flow cytometry. Data are mean ± s.d. (n = three replicates). (C) HEK293STF cells were seeded at 5k/well and treated with RNF43, ZNRF3, or RNF43-ZNRF3 fratricide REULR molecules after 24 h in the presence of 10% conditioned WNT3a media (monomeric binding moieties or PBS were used as negative controls). After 36 h, the activation of the β-catenin-dependent STF reporter by fratricide REULRs was measured. Data are mean ± s.d. (n = three replicates).