Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 24;29:58. doi: 10.1186/s10020-023-00631-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — 4-OI attenuates LPS-induced mitochondrial ROS generation, and inflammation enhances mitophagy and subsequent apoptosis, which were partially abolished by the Nrf2 inhibitor. (A) Representative western blot bands of Nrf2, HO-1, NQO1, NLRP3, and cleaved caspase-3 in BUMPT cells. (B) Relative band intensity of Nrf2, HO-1, NQO1, NLRP3 and cleaved caspase-3 in BUMPT cells (n = 4). (C) Representative IF staining images of Nrf2 (red) in BUMPT cells. Scale bar: 20 μm. (D) Representative images of MitoSOX staining of BUMPT cells. Scale bar: 20 μm. (E) Representative western blot bands of Nrf2, HO-1, NQO1, IL-1β, and cleaved caspase-3 in murine kidneys. (F) Relative band intensity HO-1, NQO1, IL-1β and cleaved caspase-3 in murine kidneys (n = 6). (G) qPCR analysis of mRNA of Il6 in renal tissues (n = 6). (H) qPCR analysis of mRNA of Lcn2 in renal tissues (n = 6). (I) Representative western blot bands of LC3B-II, p62, TIMM23, and TOMM20 in BUMPT cells (n = 4). (J) Representative western blot bands of LC3B-II, p62, TIMM23, and TOMM20 in renal tissues. (K) Relative band intensity of LC3B-II, p62, TIMM23, and TOMM20 in BUMPT cells (n = 4). (L) Relative band intensity of LC3B-II, p62, TIMM23, and TOMM20 in renal tissues (n = 6). Each symbol (circle) represents an independent experiment. Data are presented as means ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant