FIG 4.

Disruption of glycogen metabolism impacts fitness during in vitro starvation. (A) Schematic depicting the experimental design. GFPy-tagged WT and dTomato-tagged glycogen metabolism mutant or revertant strains were mixed in equal proportions and subjected to long-term starvation in YNB lacking a carbon source. At days 0, 5, 7, 10, 12, and 14 post-inoculation, aliquots were removed, density-adjusted, and plated onto YPD medium. Fluorescence images of the agar plates were captured using an IVIS Spectrum imager. (B) Fluorescent CFU were enumerated from the images, and those exhibiting red fluorescence were reported as a percentage of the total. The data are depicted as the mean ± SEM. A multiple t test was used to compare each mutant and revertant percentage at each time point. *, P < 0.05; **, P < 0.01; ***, P < 0.001.