FIG 5.

Glycogen synthase, glycogen phosphorylase, and glucoamylase mutants are less competitively fit during vaginal colonization. (A) Groups of mice (n = 4) were inoculated with mixed cultures of GFPy-tagged WT or dTomato-tagged glycogen metabolism mutant or revertant strains. At day 0 (inoculum), 4, 8, 12, and 16 (lavage) samples were plated onto YPD agar plates containing 50 μg/mL chloramphenicol, and fluorescent CFU were enumerated via IVIS imaging. Strains are depicted in the figure legend. The data are expressed as the percentage of red fluorescent colonies that were recovered and are depicted as the mean ± SEM. A multiple t test was used to compare each mutant and revertant percentage at each time point. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (B) CFU obtained from vaginal lavage samples that were obtained at days 4, 8, 12, and 16 were enumerated. The line represents the median. A multiple t test was used to compare each mutant and revertant percentage at each time point. *, P < 0.05.