FIG 3.

tRNA halves accumulate during encystation of E. invadens. (A) Small-RNA sequences generated from size selected libraries from E. invadens parasites in different stages: trophozoites, 24-h cysts, 72-h cysts, or excysting parasites at 8 h. The chart shows percentage of reads mapping to tRNA. (B) Length distribution of reads mapping to tRNA sequences. (C) Frequency of reads mapping to most abundant tRNAs. (D) Northern blot analysis of total RNA from 24-h cysts and trophozoites probed for tRNA halves. Both 5′ and 3′ halves accumulated in the early cyst stage, in contrast to the trophozoites.