FIG 5.
The M1-CovS-WT strain survives better than the M1-CovS-Y39H mutant strain inside macrophages. A macrophage survival assay was performed with mouse RAW264.7 macrophages infected with M1-CovS-WT (closed circles) and M1-CovS-Y39H (open squares) at an MOI of 10. GAS survival is expressed as a percentage of intracellular bacteria at T2, T4, and T6 relative to those at T0. The results are representative of data from 7 independent experiments performed in triplicate. Error bars represent the means and standard errors of the means (**, P < 0.01).